Kenji Tsuge (Kobe University)
Fri 28 Oct 2016, 12:30 - 13:00
C.H Waddington Building, Seminar room 1.08, King's Building's

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Along with recent great development in synthetic biology era, demand for de novo designed long DNA with several dozen kb in size is getting greater. To meet the demand, efficient DNA fragment assembly method, named 2nd generation OGAB, have been developed. This method uses plasmid transformation of Bacillus subtilis and is feasible for assemble over 50 DNA fragments at once. Using plasmid DNAs that clone short (~1 kb) synthetic DNA as material for assembly, this method is able to construction of 50 kb long DNA in short time. This method requires certain degree of skill, we therefore have been trying to construct automation system to perform this method.